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Lack of single-strand DNA-binding protein amplification under conditions of SOS induction in E. coli

✍ Scribed by Salles, Bernard ;Paoletti, Claude ;Villani, Giuseppe

Publisher: Springer
Year: 1983
Tongue: English
Weight: 257 KB
Volume: 189
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00326074

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✦ Synopsis

A two site immunoradiometric assay (IRMA) for quantification of the recA protein has been recently described (Paoletti et al. 1982). We have used a similar technique to monitor the possible amplification of the ssb protein in E. coli after induction of the SOS repair process by various DNA damaging agents. Under these conditions, while we have been able to detect a full amplification of recA protein, we failed to observed any amplification of the ssb protein.

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