In modern biology, there is a critical need to develop a high-throughput and inexpensive platform for DNA sequencing. Pyrosequencing is a nonelectrophoretic single-tube DNA sequencing method that takes advantage of cooperativity between four enzymes to monitor DNA synthesis. In these studies, single
The role of E. coli single-stranded DNA binding protein in DNA metabolism
โ Scribed by John W. Chase
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 612 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0265-9247
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โฆ Synopsis
Single-stranded DNA binding proteins have been known for some time to be crucial in many D N A metabolic reactions in both prokaryotes and eukaryotes. Despite a wealth of studies on these proteins we still do not understand their biochemical mechanism of action. Recent studies of the
Escherichia coli singlestranded DNA binding protein (SSB) are beginning to provide some insight into how this and similar proteins might function.
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Transcription in E. coli is often controlled by the binding of specific gene-regulatory proteins. Binding of these proteins to their specific DNA binding sites occurs in the presence of a large excess of "nonspecific" genomic DNA. Binding to a specific DNA site thus depends on the concentration of r