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Lack of lactate-proton symport activity in pck1 mutants of Saccharomyces cerevisiae

✍ Scribed by Margarida Casai; Miguel A. Blázquez; Francisco-javier Gamo; Carlos Gancedo; Cecília Leão

Book ID
109320588
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
341 KB
Volume
128
Category
Article
ISSN
0378-1097

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Low ribonuclease activity in cellular ly
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✍ Venkov, Pencho V. 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 189 KB

Cellular lysates with very low total ribonuclease activities are obtained by lysis of Saccharomyces cerevisiae VY1160 osmotic sensitive mutant cells in 1% sorbitol solution. These lysates could be used for isolation of intact polysomes and messenger RNA molecules, or for studying of specific ribonuc