Low ribonuclease activity in cellular lysates of osmotic sensitive Saccharomyces cerevisiae mutants

✍ Scribed by Venkov, Pencho V.

• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 189 KB
• Volume: 175
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00267862

✦ Synopsis

Cellular lysates with very low total ribonuclease activities are obtained by lysis of Saccharomyces cerevisiae VY1160 osmotic sensitive mutant cells in 1% sorbitol solution. These lysates could be used for isolation of intact polysomes and messenger RNA molecules, or for studying of specific ribonucleases.