Labeling of poly(A) associated RNA in synaptosomes and the other subcellular fractions of rat cerebral cortex in basal conditions and during training

✍ Scribed by Dr. Aroldo Cupello; Holger Hyden

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 282 KB
• Volume: 8
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490080402

✦ Synopsis

Abstract

The labeling of total and poly(A) associated RNA from the subcellular fractions of cerebral cortex was studied after a 1‐hour ^3^H‐uridine pulse in control and trained rats. In the control animals, the fraction of newly synthesized brain cortex RNA which is found in synaptosomes is the minimal one. However, in synaptosomes the proportion of poly(A)^+^ RNA within newly synthesized RNA is maximal. Training of the rats does not influence total incorporation of label into RNA in the brain cortex and the distribution of newly synthesized RNA within the subcellular fractions. The proportions of poly(A)^+^ RNA within total labeled RNA in the various fractions are also unaffected.