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L-iduronidase in cultured human fibroblasts and liver

โœ Scribed by Reuben Matalon; J.A. Cifonelli; Albert Dorfman


Book ID
118852421
Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
694 KB
Volume
42
Category
Article
ISSN
0006-291X

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On DEAE cellulose column chromatography, alpha-L-iduronidase in cultured skin fibroblasts was resolved into two distinct components, forms A and B. They had similar Km values for 4-methylumbelliferyl-alpha-L-iduronide, but differed in pH optima and thermal stability. Form B was more heat-stable than