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α-l-Iduronidase activity in cultured skin fibroblasts and amniotic fluid cells

✍ Scribed by Clara W. Hall; Elizabeth F. Neufeld

Book ID
115701912
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
418 KB
Volume
158
Category
Article
ISSN
0003-9861

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✍ S. Fujibayashi; R. Minami; Y. Ishikawa; K. Wagatsuma; T. Nakao; S. Tsugawa 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 324 KB

On DEAE cellulose column chromatography, alpha-L-iduronidase in cultured skin fibroblasts was resolved into two distinct components, forms A and B. They had similar Km values for 4-methylumbelliferyl-alpha-L-iduronide, but differed in pH optima and thermal stability. Form B was more heat-stable than