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L-Carnitine uptake by Escherichia coli

✍ Scribed by Heinrich Jung; Kirsten Jung; Hans-Peter Kleber

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
425 KB
Volume
30
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

The uptake of L‐carnitine by __Escherichia coli__044 K 74 which is able to metabolize L‐carnitine to γ‐butyrobetaine under anaerobic conditions was studied. The uptake system of E. coli was induced in the presence of L‐carnitine or crotonobetaine. The optimum influx of L‐carnitine was found to take place at 37 °C in phosphate buffer, pH 7.5. On the basis of the temperature dependence of the uptake rate an activation energy of 53.5 kJ/mol was estimated. The transport of L‐carnitine was energy dependent. The kinetics of L‐carnitine entry followed the MICHAELIS‐MENTEN relationship yielding a K~m~ value of 5.3 · 10^‐4^ M and a V~max~ of 154 nmol/min · mg protein. The transport system showed considerable structural specifity for trimethylamine carboxylic molecules. The results suggest that E. coli possesses an inducible, active and carrier‐mediated uptake system for L‐carnitine.

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