Kluyveromyces marxianus: a potential source of diacetyl reductase

✍ Scribed by J.G. Schwarz; Y.D. Hang

• Book ID: 104640733
• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 235 KB
• Volume: 10
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00144456

✦ Synopsis

Kluyoeromyces mamianus had a higher specific activity of diacetyl reductase (EC 1.1.1.5) than all other organisms previously reported. The enzyme was NADH-dependent and irreversibly catalysed the conversion of diacetyl to acetoin with an optimum pH of 7.0. It was stable at 40°C but lost 50% of its activity at 50°C in 30 min. The K,,, and V,,, values for diacetyl were 1.8 mM and 0.053 mM/min, respectively.