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Kinetics of α-amylase synthesis from Bacillus amyloliquefaciens

✍ Scribed by Young J. Yoo; Theodore W. Cadman; Juan Hong; Randolph T. Hatch

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
548 KB
Volume
31
Category
Article
ISSN
0006-3592

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✦ Synopsis

The microbial production of a-amylase from Bacillus amyloliquefaciens was investigated. The microorganism was grown using media containing glucose or maltose at 37°C and under aerobic conditions in a 16-L fermentor. The a-amylase synthesis from maltose was not found to be inducible but was found to be subject to catabolite repression. The maltose uptake rate was observed to be the rate-limiting step compared to the conversion rate of maltose to glucose by intracellular a-glucosidase. The a-amylase activity achieved with maltose a s a substrate was higher than that achieved with glucose. A slower growth rate and a higher cell density were obtained with maltose. The enzyme production pattern depended upon the nutrient composition of the medium.

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