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Kinetics of α-amylase secretion in Aspergillus oryzae

✍ Scribed by Anne L. Santerre Henriksen; Morten Carlsen; Henriette de Bang; Jens Nielsen

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
487 KB
Volume
65
Category
Article
ISSN
0006-3592

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✦ Synopsis

Pulse and pulse-chase experiments have been performed to study L-[ 35 S] methionine incorporation and protein secretion kinetics in Aspergillus oryzae. Pulse experiments confirmed the mechanism of methionine uptake reported previously for Penicillium chrysogenum (Benko et al., 1967). Pulse-chase experiments were carried out to investigate the ␣-amylase secretion kinetics in A. oryzae. No unglycosylated ␣-amylase was detected neither intracellularly nor extracellularly demonstrating that glycosylation was not the rate controlling step in the secretory pathway. The pulse chase experiments indicated that there are two pools of intracellular ␣-amylase: a fast secreted and a slow secreted. The secretion of those two pools were described with a kinetic model, which was fitted to the pulse chase experiments.

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