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Kinetics of the Yeast Cystathionine β-Synthase Forward and Reverse Reactions: Continuous Assays and the Equilibrium Constant for the Reaction †

✍ Scribed by Aitken, Susan M.; Kirsch, Jack F.


Book ID
121801816
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
122 KB
Volume
42
Category
Article
ISSN
0006-2960

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A continuous, coupled, spectrophotometric assay is described in which the enzyme ATP sulfurylase is employed to measure the concentration of inorganic pyrophosphate (PPi) at equilibrium with known concentrations of inorganic orthophosphate (Pi) in the presence of excess inorganic pyrophosphatase (PP