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Kinetics of the micellar nucleophillic cleavage of diastereomeric phosphotriesters

โœ Scribed by Robert A. Moss; Susmita Bose


Publisher
Elsevier Science
Year
1997
Tongue
French
Weight
190 KB
Volume
38
Category
Article
ISSN
0040-4039

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An assay to determine the kinetics of RN
โœ Robert Corcoran; Marc Labelle; Anthony W. Czarnik; Ronald Breslow ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 530 KB

To evaluate some synthetic catalysts that mimic ribonuclease, a quantitative assay has been developed that measures the number of phosphate diester bonds cleaved in a polymeric RNA substrate. This assay involves determining the number of 5'-oligonucleotide termini produced during the cleavage, using