Kinetics of phenolic polymerization catalyzed by peroxidase in organic media

✍ Scribed by Dr. Yue-Ping Xu; Guo-Lan Huang; Yao-Ting Yu

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 274 KB
• Volume: 47
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260470114

✦ Synopsis

Phenolic polymerization was carried out by enzymatic catalysis in organic media, and its kinetics was studied by using high-pressure liquid chromatography (HPLC). Phenols and aromatic amines with electron-withdrawing groups could hardly be polymerized by HRP catalysis, but phenols and aromatic amines with electron-donating groups could easily be polymerized. The reaction rate of either the para-substituted substrate or meta-substituted substrate was higher than that of ortho-substituted substrate. When ortho-position of hydroxy group of phenols was occupied by an electron-donating group and if another electron-donating group occupied para-position of hydroxy group, the reaction rate increased. Horseradish peroxidase and lactoperoxidase could easily catalyze the polymerization, but chloroperoxidase and laccase failed to yield polymers. Metallic ions such as Mn2+, Fe2+, or Fe3+, and Cu2+ could poison horseradish peroxidase to various extents, but ions such as Co2+, Cd2+, Zn2+, and K+ were not found to inhibit the reaction.