Kinetics of Enzymes with Iso-Mechanisms: Solvent Isotope Effects
β Scribed by Dexter B. Northrop; Karen L. Rebholz
- Book ID
- 115566541
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 103 KB
- Volume
- 342
- Category
- Article
- ISSN
- 0003-9861
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## Abstract The basic principles and illustrative applications to enzyme mechanisms of solvent isotope effects, with emphasis on the use of rate measurements in mixtures of protium and deuterium oxides (βproton inventoriesβ), are reviewed over a period slightly shorter than the history of this Jour
## Abstract Solvent isotope effects have been observed on the in vitro refolding kinetics of a protein, hen lysozyme. The rates of two distinct phases of refolding resolved by intrinsic fluorescence have been found to be altered, to differing extents, in D~2~O compared with H~2~O, and experiments h