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Kinetics of DNA and RNA Hybridization in Serum and Serum-SDS

✍ Scribed by Graugnard, E.; Cox, A.; Jeunghoon Lee; Jorcyk, C.; Yurke, B.; Hughes, W.L.

Book ID
115494420
Publisher
IEEE
Year
2010
Tongue
English
Weight
459 KB
Volume
9
Category
Article
ISSN
1536-125X

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The rates of duplex formation for two octamers of DNA (5β€² d-CACGGCTC/5β€² d-GAGCCGTG and 5β€² d-CACAGCAC/5β€² d-GTGCTGTG), the homologous RNA, and both sets of hybrids in 1 M NaCl buffer have been measured using stopped-flow spectroscopy. In addition, the thermodynamic parameters, Ξ”HΒ°and Ξ”SΒ°, have been de