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Kinetics of Conjugative Transfer: A Study of the Plasmid pXO16 fromBacillus thuringiensissubsp.israelensis

โœ Scribed by Lars Andrup; Lasse Smidt; Katja Andersen; Lars Boe


Book ID
112263416
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
406 KB
Volume
40
Category
Article
ISSN
0147-619X

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The oriT site of the broad host-range multicopy IncQ plasmid RSF1010 was cloned onto the 2.2 kb pBR322-derived vector pED825. By successive subcloning and construction of deletions, the oriT region was localised on an 80-88 bp segment of DNA. This segment was contained within the HaeII fragment of R