Kinetics of baculovirus replication and release using real-time quantitative polymerase chain reaction

✍ Scribed by Matthew Rosinski; Steven Reid; Lars Keld Nielsen

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 109 KB
• Volume: 77
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.10126

✦ Synopsis

Abstract

The study of viral‐based processes is hampered by (a) their complex, transient nature, (b) the instability of products, and (c) the lack of accurate diagnostic assays. Here, we describe the use of real‐time quantitative polymerase chain reaction to characterize baculoviral infection. Baculovirus DNA content doubles every 1.7 h from 6 h post‐infection until replication is halted at the onset of budding. No dynamic equilibrium exists between replication and release, and the kinetics are independent of the cell density at the time of infection. No more than 16% of the intracellular virus copies bud from the cell. © 2002 John Wiley & Sons, Inc. Biotechnol Bioeng 77: 476–480, 2002; DOI 10.1002/bit.10126