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Kinetics of acrylamide/glutathione binding by fast atom bombardment and thermospray mass spectrometry

✍ Scribed by M. E. Harrison; M. A. Baldwin

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 332 KB
Volume: 24
Category: Article
ISSN: 1076-5174
DOI: 10.1002/oms.1210240828

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✦ Synopsis

The kinetics of the binding of the neurotoxin acrylamide to the cysteine residue of glutathione has been studied. At 37°C and pH 7.3 the second order rate constant has been determined to be 0.72 f 0.06 mol-' dm3 min-' by thermospray mass spectrometry. The critical energy at pH 115 measured over the temperature range 10-37 "C by fast atom bombardment mass spectrometry was measured as 24.6 kJ mol-'.

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