Fast atom bombardment mass spectrometry and fast atom bombardment mass spectrometry/mass spectrometry of three glutathione conjugates of acetaminophen

✍ Scribed by J. O. Lay Jr.; D. W. Potter; J. A. Hinson

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 382 KB
• Volume: 14
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200140907

✦ Synopsis

Three glutathione conjugates of acetaminophen were characterized by fast atom bombardmentlmass spectrometry (FABlMS) and fast atom bombardmentlmass spectrometryhass spectrometry (FABlMSlMS). The conjugates, 3-(glutathion-S-yl)acetaminophen, 3-(glutathion-S-yl)diacetaminophen and 3-(diglutathion-S-yl)diacetaminophen showed intense [MH]+ ions at m l z 457, 606 and 911, respectively. Only 3-(glutathion-S-yl)acetaminophen showed any fragmentation by FABIMS. Structurally characteristic fragmentation was observed with all three conjugates when the [MH]+ ions were collisionally activated. The loss of the glycine (GLY) and glutamic acid (GLU) moieties indicated the presence of at least one glutathione (GSH) group. Multiple losses, some of which could only occur via cleavages in both GSH moieties, were observed with the diglutathione conjugate.