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Kinetics and specificity of serine proteases in peptide synthesis catalyzed in organic solvents

✍ Scribed by Hubert GAERTNER; Antoine PUIGSERVER


Book ID
115126250
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
747 KB
Volume
181
Category
Article
ISSN
1432-1327

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Calculation of kinetic constants of an enzymatic reaction in organic solvents requires knowledge of the functional active-site concentration in organic solvents, and this can be significantly different than that in water. An experimental method for active-site titration of serine proteases in organi