Kinetics and modeling of hexavalent chromium reduction in enterobacter cloacae

✍ Scribed by Koji Yamamoto; Junichi Kato; Takuo Yano; Hisao Ohtake

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 461 KB
• Volume: 41
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260410117

✦ Synopsis

Kinetics of bacterial reduction of toxic hexavalent chromium (chromate: Cr04'-) was investigated using batch and fedbatch cultures of Enterobacter cloacae strain H01. In fedbatch cultures, the Cr04'-feed was controlled on the basis of the rate of pH change. This control strategy has proven to be useful for avoiding toxic Cr0; -overload. A simple mathematical model was developed to describe the bacterial process of Cr04'-reduction. In this model, two types of bacterial cells were considered: induced, CrO,'--resistant cells and uninduced, sensitive ones. Only resistant cells were assumed to be able to reduce CrO,,-. These fundamental ideas were supported by the model predictions which well approximated all experimental data. In a simulation study, the model was also used to optimize fed-batch cultures, instead of lengthy and expensive laboratory experiments.