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Kinetics and mechanism of acyl transfer by penicillin G acylase comparing acyl donors methyl chloroacetate and methyl phenylacetate

✍ Scribed by Kouji Isaka; Naoko Suga; Fumihiro Ishimura

Book ID: 113397515
Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 622 KB
Volume: 79
Category: Article
ISSN: 0922-338X
DOI: 10.1016/0922-338x(95)90607-2

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## Abstract The hypothesis that at least some penicillin acylases react via an acyl–enzyme intermediate was tested by kinetic investigations involving the methyl ester of cyanoacetic acid as the acyl donor, deacetyl‐7‐aminocephalosporanic acid as the acyl receptor, and penicillin acylase (E.C.3.5.1