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Kinetically controlled urease electrode

✍ Scribed by H. Hamann; F. Scheller; M. Kühn

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
356 KB
Volume
1
Category
Article
ISSN
1040-0397

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✦ Synopsis

The parameters and the measuring principle of a urease electrode are described. The urease is physically entrapped in cellulose triacetate on the active surface of a pH glass electrode with an internal reference electrode. The Michaelis constant of immobilized urease was similar to that of soluble enzyme (KM = 2.40 mmol/L). The average apparent enzyme activity amounts to 2 IU per sensor preparation. An enzyme loading factor of 0.04 was determined. It indicates kinetically controlled reaction conditions of the sensor. A fixed-time measuring procedure provides a response time of 30 seconds and a linear response within a concentration range of 2 to 40 mmol/L urea in real biological samples (e.g., serum).

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