๐”– Bobbio Scriptorium
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Electrode-based enzyme immunoassays using urease conjugates

โœ Scribed by M.E. Meyerhoff; G.A. Rechnitz

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
888 KB
Volume
95
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Urease conjugates are employed for competitive-binding enzyme immunoassays (EIA) of a model antigen, bovine serum albumin (BSA), and of cyclic AMP (CAMP). Urease activity bound to a double-antibody solid phase is determined with an ammonia gassensing electrode, after appropriate washing steps. Cyclic AMP analogs coupled to urease are used to determine their effect on the overall response characteristics of the CAMP assay. The use of urease as a label for EIA purposes is shown to yield sensitive assays for both proteins (BSA < 10 t&ml) and haptens (CAMP < lo-* M) with good day-to-day reproducibility.

' Abbreviations used: EIA, enzyme immunoassay; B(H)SA. bovine (human) serum albumin.

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