Catalytic reactions of DNA polymerase I from E. coli (Klenow fragment, KF) were monitored directly with a template/primer (40/25-or 75/25-mer)immobilized 27-MHz quartz-crystal microbalance (QCM). The 27-MHz QCM is a very sensitive mass-measuring device in aqueous solution, as the frequency decreases
โฆ LIBER โฆ
Kinetic studies on DNA polymerase
โ Scribed by D. Beyersmann; G. Schramm
- Book ID
- 115731791
- Publisher
- Elsevier Science
- Year
- 1968
- Weight
- 535 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0005-2744
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These studies have been conducted as a first step toward investigating the molecular mechanism by which DNA-dependent RNA polymerase recognizes a specific region of DNA. The DNA moieties are either bound to or freed from RNA polymerase. Results obtained so far indicate that the DNA moieties remainin