✦ LIBER ✦

Kinetic modelling of liposome degradation in peritoneal macrophages

✍ Scribed by Hideyoshi Harashima; Noriko Hirai; Hiroshi Kiwada

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 608 KB
Volume: 16
Category: Article
ISSN: 0142-2782
DOI: 10.1002/bdd.2510160206

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The objective of this study was to quantify and model the degradation process of liposomes in peritoneal macrophages (PMs). Iodinated albumin (^125^I‐alb) was chosen to be the marker of liposome degradation. The time course of the degradation of free ^125^I‐alb after pinocytosis by PMs followed first‐order kinetics with a half‐life of 23 min. The degradation of liposomally encapsulated ^125^I‐alb was also quantified. Kinetic modelling of liposome degradation indicated the existence of two kinetically different processes, one with a half‐life of 13 min and the other with a half‐life of 7.5 h. Comparing the degradation of liposomal and free ^125^I‐alb suggested that ^125^I‐alb was delivered to lysosomes much faster through phagocytosis than pinocytosis. These results indicate that the intracellular degradation kinetics of pinosomes and phagosomes is different. This method can quantify the rate and extent of liposomal degradation in macrophages and provide kinetic information on the intracellular destiny of liposomally encapsulated compounds.

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