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Kinetic characterization of recombinant human cystathionine β-synthase purified from E. coli

✍ Scribed by Muluken S. Belew; Faraz I. Quazi; William G. Willmore; Susan M. Aitken

Book ID
116828914
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
355 KB
Volume
64
Category
Article
ISSN
1046-5928

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✍ Sylvie Blond; Dr. Michel E. Goldberg 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 841 KB

This report describes the use of fluorescence energy transfer between an intrinsic energy donor (tryptophan 177) and two chemically added acceptors to study intermediates in the folding of the beta 2 subunit of E. coli tryptophan-synthase. Two early folding steps are thus identified and characterize