Kinetic behaviour of immobilized enzyme membrane reactors
✍ Scribed by Francesco Afani au[Gabriele Iorio; Guido Greco Jr.; Maria Cantarella; Marie Hélène Remy; Vincenzo Scardi
- Book ID
- 103007522
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 696 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0009-2509
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## Abstract A flat‐membrane dialyzer was used as enzyme reactor by introducing enzyme solution into one of the membrane‐separated chambers. The apparent Michaelis constant __K__~__m__~(app) of urease was always larger (ten times at [urease] = 1 mg/ml) than that of free enzyme because the permeation
Glucose oxidase from A. niger (EC 1.1.3.4) was physically entrapped between two dialysis membranes. The resultant enzyme-membrane sandwich reactors were employed in a standard Technicon dialyzer unit at 37°C and were found to perform efficiently in the automated analysis of aqueous glucose standards