Kinetic analysis of hybridoma cell culture in a protein-free medium: Substrate and agitation effects
✍ Scribed by Lorea Legazpi; Jaime Díaz; Adriana Laca; Mario Díaz
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 463 KB
- Volume
- 26
- Category
- Article
- ISSN
- 1369-703X
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## Abstract The steady‐state metabolic parameters for a hybridoma cell line have been determined in continuous suspension–perfusion culture over a wide range of perfusion rates and cell bleed rates. Significant increases in viable cell concentrations and volumetric productivities were achieved at h
BiP, GRP94 and PDI, three endoplasmic reticulum (ER) based proteins are involved in the maturation of secretory proteins and might represent a bottleneck in the secretory pathway of monoclonal antibodies (MAB). With the three hybridoma cell lines tested, MAB production kinetics were significantly in