Potentiometric enzyme electrodes for urea, AMP, and adenosine have been used for the kinetic assays of arginase (EC 3.5.3.1), 3':5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17), and 5'-nucleotidase (EC 3.1.3.5), respectively. The initial rate of potential change, after addition of the enzyme to
โฆ LIBER โฆ
Kinetic analysis of enzyme electrode response
โ Scribed by Bradley, C. R.; Rechnitz, G. A.
- Book ID
- 127158886
- Publisher
- American Chemical Society
- Year
- 1984
- Tongue
- English
- Weight
- 472 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0003-2700
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An explmt pomt method IS described for dwtal snnulatlon of redox-enzyme catalysis and diffislon at soluble-enzyme electrodes Two experImenta conchtlons (chronopotentlometry and open-arcmt potential decay) were sunulated for a two-substrate enzyme (glucose oxldase) catalyzing the anaerobic oxidation