KIAA0369, doublecortin-like kinase, is expressed during brain development

✍ Scribed by Harold A. Burgess; Salvador Martinez; Orly Reiner

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 242 KB
• Volume: 58
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/(sici)1097-4547(19991115)58:4<567::aid-jnr9>3.0.co;2-t

✦ Synopsis

During embryonic development, the cerebral cortex attains its characteristic adult laminated structure. The finding that X-linked lissencephaly patients harbor mutations in the doublecortin gene implicated this gene product in the process of corticogenesis. An autosomal human gene, KIAA0369, with a high level of similarity to doublecortin, has been cloned from human adult brain. This gene product contains a kinase domain in addition to a doublecortin-like domain. In order to evaluate whether this doublecortinlike kinase also plays a role during brain development, we cloned and studied the expression pattern of the mouse homolog. Three cDNA products of this gene were cloned: one, doublecortin-like kinase, the second containing only the doublecortin-like region, and the third containing only the kinase domain, a homolog of the previously cloned rat CPG16 gene. We studied doublecortin-like kinase expression in mouse using Northern blot analysis, in situ hybridization, and Western blot analysis, and conclude that doublecortinlike kinase is expressed in multiple regions of embryonic brain including the developing cerebral cortex. J.