Keratinocyte growth-promoting activity from human placenta

✍ Scribed by E. J. O'Keefe; R. E. Payne JR.; N. Russell

• Book ID: 102880468
• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 829 KB
• Volume: 124
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041240312

✦ Synopsis

Extracts of term human placenta were tested for enhancement of proliferative growth of primary cultures of human keratinocytes. Saline extracts or supernatants from homogenates were dialyzed extensively, lyophilized, and tested in subcultures of keratinocytes in MCDB 153 medium with 0.1 mM Ca++ containing only defined supplements (insulin, hydrocortisone, transferrin, ethanolamine, phosphoethanolamine). Cells plated in the absence of EGF at moderately high densities (1000-3000 cells per cm2) formed colonies and grew in the presence of placental extract at 25-500 &ml. Extracts of cord serum or maternal serum were inactive, suggesting that the activity is derived from placental tissue. The activity is not EGF, since the activity in t h e placental extract, unlike ECF, did not promote growth at low cell density, was synergistic with ECF under some conditions, and did not produce changes in colonial morphology which occurred in t h e presence of EGF. Unlike keratinocyte growth-promoting activity in bovine hypothalamic extract, the activity is nondialyzable and is destroyed at 100°C. Placental extract could not replace any of the defined components of the medium and is therefore distinct from them. The presence of activity in the placenta with distinctive properties suggests that this is a previously undescribed material with growth-promoting properties for epithelium.