✦ LIBER ✦

Human keratinocyte growth-promoting activity on the surface of fibroblasts

✍ Scribed by Peter C. Yaeger; Charles D. Stiles; Barrett J. Rollins

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 944 KB
Volume: 149
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041490114

No coin nor oath required. For personal study only.

✦ Synopsis

To proliferate in serum-containing medium, normal human keratinocytes must be co-cultured with fibroblast feeder cells. Conditioned medium from feeder cell cultures cannot substitute for the cells themselves. We tested the hypothesis that fibroblasts display a keratinocyte growth-promoting activity on their outer cell surface. The results of our investigation showed that (I 1 glutaraldehyde-fixed fibroblast feeder cells promote keratinocyte growth, (2) the growth-promoting effect requires contact between fixed fibroblasts and keratinocytes, and ( 3 ) feeder activity is highly enriched within the plasma membrane fraction of fibroblasts. We conclude that at least part of the fibroblast "feeder" activity involves a keratinocyte growth-promoting factor which is bound to the outer surface of fibroblast plasma membranes.

📜 SIMILAR VOLUMES

Human keratinocyte growth factor activit

Human keratinocyte growth factor activity on proliferation and differentiation of human keratinocytes: Differentiation response distinguishes KGF from EGF family

✍ C. Marchese; A. Messina; A. Faggioni; M. R. Torrisi; L. Frati; D. Ron; J. Rubin; 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 813 KB

Human keratinocyte growth factor (KGF) is an epithelial cell specific mitogen which is secreted by normal stromal fibroblasts. In the present studies, we demonstrate that KGF is as potent as EGF in stimulating proliferation of primary or secondary human keratinocytes in tissue culture. Exposure of K

Activation of the insulin-like growth fa

Activation of the insulin-like growth factor-1 receptor promotes the survival of human keratinocytes following ultraviolet B irradiation

✍ Christine Kuhn; Steven A. Hurwitz; Manish G. Kumar; Jenny Cotton; Dan F. Spandau 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 639 KB

The ultraviolet B (UVB) component of sunlight causes non-melanoma skin cancers due to the damage it inflicts on genomic DNA. The response of epidermal keratinocytes to sunlight depends on the dose of UVB received and the severity of the damage to the DNA. Mild DNA damage typically induces DNA-repair

Keratinocyte growth arrest is associated

Keratinocyte growth arrest is associated with activation of a transcriptional repressor element in the human cdk1 promoter

✍ Alison L. Dahler; Susan J. Jones; Anthony J. Dicker; Nicholas A. Saunders 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 264 KB

In this study we examined the regulation of cdk1 expression in normal human epidermal keratinocytes (HEKs) and neoplastic keratinocytes. Keratinocytes were growth-arrested by allowing the cells to grow to confluence or by treating them with interferon-gamma (IFNg) or 12-O-tetradecanoyl phorbol-13-ac

Acrolein induces activation of the epide

Acrolein induces activation of the epidermal growth factor receptor of human keratinocytes for cell death

✍ Kei Takeuchi; Masashi Kato; Haruhiko Suzuki; Anwarul A. Akhand; Jianghong Wu; Kh 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 217 KB

## Abstract Acrolein, which is a highly reactive formaldehyde generated by lipid peroxidation, can affect skin and cause various disorders. The effect of exposure of human keratinocytes to acrolein on cell surface‐oriented signal transduction into cells was examined. Incubation of human keratinocyt

Human colon fibroblasts induce different

Human colon fibroblasts induce differentiation and proliferation of intestinal epithelial cells through the direct paracrine action of keratinocyte growth factor

✍ Vincenzo Visco; Felice A. Bava; Federica d'Alessandro; Marco Cavallini; Vincenzo 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 842 KB

## Abstract The effects exerted by the keratinocyte growth factor (KGF) on intestinal epithelial cells cultured in vitro are influenced by cell confluence and differentiation through the modulation of keratinocyte growth factor receptor (KGFR) expression. In order to better define the contribution

Effect of hyaluronan on the elastase-typ

Effect of hyaluronan on the elastase-type activity of human skin fibroblasts.

✍ Emmanuelle Bernard; William Hornebeck; Ladislas Robert 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 230 KB

## ABSTRACT The influence of hyaluronan (HA) on the expression of human skin fibroblast elastase‐type protease (HSFEp) (Homsy et al, 1988) was studied. At confluency of HSF cultures, hyaluronan increased the level of HSFEp in a time and dose‐dependent fashion, Optimal effect was observed after 48 h