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Jasmonic acid stimulates shoot and bulb formation of garlic in vitro

✍ Scribed by Maja Ravnikar; Jana Žel; Igor Plaper; Alenka Špacapan


Book ID
104658446
Publisher
Springer
Year
1993
Tongue
English
Weight
623 KB
Volume
12
Category
Article
ISSN
0721-7595

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✦ Synopsis


Although much information is available concerning the involvement of jasmonates in the regulation of plant development, few reports are devoted to their effects in tissue culture. In the present study, the influence of jasmonic acid (JA) on shoot and bulb formation in tissue culture of garlic (AUium sativum cv. Ptuj) was studied. Isolated basal plates were placed onto Gamborg's B5 medium. JA significantly enhanced the shoot and bulb development in concentrations from 1-10 p,M. When the combination of 10 p.M JA and 5 txM 2-iP was used in the initiation media, the average number of shoots was 30 after 6 weeks of culture. The bulbs formed on approximately 50% of shoots. Results described in this article show that JA might play an important role in the formation of storage organs in plants, in this case on garlic bulbs.


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In vitro shoot proliferation and product
✍ Yasseen Mohamed-Yasseen; Walter E. Splittstoesser; Richard E. Litz 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 921 KB

A procedure is described to regenerate shoots and bulbs in vitro with high frequency from shoot tips of garlic and shallot plants using benzyladenine or thidiazuron. Regenerated shoots were induced to form bulbs in Murashige and Skoog medium (1962) containing 5 g 1-1 activated charcoal and 120 g 1-1