Although much information is available concerning the involvement of jasmonates in the regulation of plant development, few reports are devoted to their effects in tissue culture. In the present study, the influence of jasmonic acid (JA) on shoot and bulb formation in tissue culture of garlic (AUium
In vitro shoot proliferation and production of sets from garlic and shallot
β Scribed by Yasseen Mohamed-Yasseen; Walter E. Splittstoesser; Richard E. Litz
- Book ID
- 104617362
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 921 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0167-6857
No coin nor oath required. For personal study only.
β¦ Synopsis
A procedure is described to regenerate shoots and bulbs in vitro with high frequency from shoot tips of garlic and shallot plants using benzyladenine or thidiazuron. Regenerated shoots were induced to form bulbs in Murashige and Skoog medium (1962) containing 5 g 1-1 activated charcoal and 120 g 1-1 sucrose under a long-day photoperiod. Bulbs formed in vitro were transferred to soil without acclimatization and produced viable plants. This method could be useful to produce low-cost bulbs, which are easy to handle and store until needed.
π SIMILAR VOLUMES
Shoots were produced from kurrat seedling and mature plant explants cultured in Murashige and Skoog medium (MS) alone or supplemented with 4.4 ~tM benzyladenine (BA). Shoots were also produced from explants through a two-step procedure. Regenerated shoots were induced to form roots on MS medium with
The in vitro shoot proliferation ofPopulus alba XP. grandidentata was affected by the medium consistency and shoot density, but not by three sizes of vessels. After 4 weeks of culture, the fresh weight and number of shoots per explant on liquid medium were significantly greater than those on agar-so