J chain deficiency in human IgM monoclonal antibodies produced by Epstein-Barr virus-transformed B lymphocytes
โ Scribed by Y. Gloria Meng; Anne B. Criss; Katy E. Georgiadis
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 433 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0014-2980
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A method of producing human monoclonal antibody by combining somatic cell hybridization technology with the capability of Epstein-Barr virus (EBV) to transform human B lymphocytes is described. Peripheral blood lymphocytes from a donor with positive tuberculin skin test reaction were transformed by
Human lymphocytes that produce anti-pneumococcal antibodies were separated and immortalized by Epstein-Barr virus and then cloned. One clone (NAD-Sel) produces an IgA, kappa antibody which is specific for the polysaccharides of type 8 pneumococcus, while not reactive with any of the polysaccharides