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Isozyme Ratios of Human Serum 5‘-Nucleotide Phosphodiesterase

✍ Scribed by K. C. TSOU; K. W. LO

Book ID
110860379
Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
235 KB
Volume
8
Category
Article
ISSN
0300-9475

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A rapid fluorogenic method for the demonstration of S-nucleotide phosphodiesterase in human serum has been developed. This method uses the substrate 4-methylumbelliferyl Sthymidylate impregnated in agarose gels or filter paper strips. Zymograms are developed in less than 30 min at 25°C. and the sens

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5'-nucleotide phosphodiesterase (5'-NP) isoenzymes were separated from the serum of human primary liver cancer (PLC) patients by 4-20% tubular polyacrylamide gel gradient electrophoresis. Electrophoresis showed 11 isoenzymes in which bands I, V and VI (from anode to cathode) were all absent in 125 n