✦ LIBER ✦
A fluorogenic method for the demonstration of human serum 5′-nucleotide phosphodiesterase isozymes after polyacrylamide gel electrophoresis
✍ Scribed by K.W. Lo; S. Aoyagi; K.C. Tsou
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 687 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A rapid fluorogenic method for the demonstration of S-nucleotide phosphodiesterase in human serum has been developed. This method uses the substrate 4-methylumbelliferyl Sthymidylate impregnated in agarose gels or filter paper strips. Zymograms are developed in less than 30 min at 25°C. and the sensitivity of this method has been compared with that of the indigogenic method.