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Isotope dilution assay for biotin

โœ Scribed by K. Dakshinamurti; A.D. Landman; Lila Ramamurti; R.J. Constable


Book ID
107711611
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
375 KB
Volume
61
Category
Article
ISSN
0003-2697

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Using glycerol kinase and [3H]glycerol, a kinetic isotope dilution assay for glycerol has been developed. Reactant and product are separated by stepwise elution from QAE-Sephadex. This assay is sensitive to as little as 100 pmol of glycerol, avoids numerous drawbacks of the traditional fluorescent a