Dihydrofolate reductase was synthesized in a batch system in the presence of the af6Nty ligand methotrexate, bound to various matrices. l k o types of gel were used: commercial methotrexate-agarose with pores inaccessible for translation machinery and methotrexate-POROS with pores easily accessible
Isotachophoretic analysis of the dihydrofolate reductase reaction in the presence of methotrexate and ascorbic acid
✍ Scribed by Susanne Renner; Zyrafete Kuçi; Hubert d'Cruze; Dietrich Niethammer; Gernot Bruchelt
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 141 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0173-0835
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📜 SIMILAR VOLUMES
We have studied the rate of transcription of t h e gene for dihydrofolate reductase (DHFR) in mouse 3T6 fibroblasts during serum-induced transitions between the resting (Go) and growing states. As a model system, we have used a methotrexate-resistant 3T6 cell line that overproduces DHFR and its mRNA
## Abstract A simple sensor was obtained through __N__‐isobutyryl‐L‐(D)‐cysteine enantiomers self‐assembled monolayer. It was demonstrated that the __N__‐isobutyryl‐cysteine modified gold electrodes can enantioselectively recognize 3,4‐dihydroxyphenylalanine (dopa) enantiomers. The electrocatalytic