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Isotachophoresis of nucleic acids in agarose gel rods

✍ Scribed by V. N. Kondratova; I. V. Botezatu; V. P. Shelepov; A. V. Lichtenstein


Book ID
110155269
Publisher
Springer
Year
2009
Tongue
English
Weight
193 KB
Volume
74
Category
Article
ISSN
0006-2979

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A very simple method is described which allows the separation of DNA molecules in a wide molecular weight range (from 0.6 to about \(30 \mathrm{~kb}\) ) in the same electrophoresis agarose gel. This is based on the achievement of a voltage gradient through a simple device consisting of a Plexiglas p