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Isolation, sequencing, and characterization ofcrp-5, a gene encoding aNeurosporaribosomal protein

✍ Scribed by Zihua Wang; Khaled A. Tarawneh; Stephen J. Free

Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 553 KB
Volume: 23
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00310894

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✦ Synopsis

A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences showed that they contain an open reading frame which encodes a protein homologous to the rat ribosomal protein S26. The crp-5 mRNA levels are regulated in a carbon-source-dependent manner. The organization of the gene and the region upstream of the coding sequences are discussed.

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