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Isolation, Purification, and Characterization of Two Thermostable Endo-1,4-β-d-glucanase Forms fromOpuntia vulgaris

✍ Scribed by Sivalingam Shyamala; Sambandam Ravikumar; Jeyaraman Vikramathithan; Kotteazeth Srikumar

Book ID
107566015
Publisher
Springer-Verlag
Year
2011
Tongue
English
Weight
391 KB
Volume
165
Category
Article
ISSN
0273-2289

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✍ Kunio Ohmiya; Keiko Maeda; Shoichi Shimizu 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 875 KB

An enzyme active against 0-(carboxymethyl)cellulose (CMC) was purified from a synthetic medium containing ball-milled cellulose wherein Ruminococcus albus had been cultivated for 70 h. After 570-fold purification, a homogeneous enzyme was obtained in a yield of 3%. The enzyme degraded CMC (molecular