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Isolation of virally-infected insect cells from a population containing infected and uninfected cells

โœ Scribed by Alexander Matschiner; Jonathan S. Dordick; David W. Murhammer


Book ID
104648251
Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
359 KB
Volume
9
Category
Article
ISSN
0951-208X

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โœฆ Synopsis


A method was developed that can be used to isolate virally-infected insect cells from a mixed population containing infected and uninfected cells. Spekfkally, Spodopteru frugiperda Sf-9 cells infected with the Autographa californica multiple nuclear polyhedrosis virus were treated with a primary antibody specific for the gp64 protein present .on the surface of virally-infected cells and a secondary antibody labeled with a fluorochrome. The resulting labeled cells were isolated by using fluorescence-activated cell sorting.


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