Isolation of virally-infected insect cells from a population containing infected and uninfected cells

✍ Scribed by Alexander Matschiner; Jonathan S. Dordick; David W. Murhammer

• Book ID: 104648251
• Publisher: Springer-Verlag
• Year: 1995
• Tongue: English
• Weight: 359 KB
• Volume: 9
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00158543

✦ Synopsis

A method was developed that can be used to isolate virally-infected insect cells from a mixed population containing infected and uninfected cells. Spekfkally, Spodopteru frugiperda Sf-9 cells infected with the Autographa californica multiple nuclear polyhedrosis virus were treated with a primary antibody specific for the gp64 protein present .on the surface of virally-infected cells and a secondary antibody labeled with a fluorochrome. The resulting labeled cells were isolated by using fluorescence-activated cell sorting.