HIV-1 proviral DNA was detected by the polymerase chain reaction (PCR) in leucocytes from the peripheral blood (PBL) and bronchoalveolar lavage (BAL) of 100% and 72%, respectively, of HIV-1 seropositive patients. Infective virus isolated by cocultivation was recovered from the PBL and BAL of 50% and
Isolation of variants of lymphocytopathic retroviruses from the peripheral blood and cerebrospinal fluid of patients with ARC or AIDS
✍ Scribed by Dr. H. Rübsamen-Waigmann; W. B. Becker; E. B. Helm; R. Brodt; H. Fischer; K. Henco; H. D. Brede
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 916 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0146-6615
No coin nor oath required. For personal study only.
✦ Synopsis
LAV/HTLV-III/AAV viruses were isolated from 20 German patients with ARC/ AIDS in order to investigate strain variation. Virus was isolated from the peripheral blood and/or cerebrospinal fluid (CSF) in umbilical cord peripheral blood lymphocyte (PBL) cultures. Isolates were identified by their cytopathic effect (CPE), by reverse transcriptase assays on cell-free infected culture supernatant fluid (SNF), and one or more of the following: immunofluorescence assays on
📜 SIMILAR VOLUMES
The cerebrospinal fluid (CSF) represents an important source of T lymphocytes that could be involved in the inflammatory response occurring in the central nervous system in multiple sclerosis (MS). In order to investigate whether the Vbeta gene usage of CSF T lymphocytes is restricted, we analyzed t
Polymorphonuclear leukocytes (PM Ns) isolated from peripheral blood and synovial fluid o f patients with rheumatoid arthritis and from peripheral blood o f volunteers were stimulated with 12-phorbol-13-myristate acetate (PMA). No significant differences in luminol-amplified chemiluminescence were fo