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Isolation of variants of lymphocytopathic retroviruses from the peripheral blood and cerebrospinal fluid of patients with ARC or AIDS

✍ Scribed by Dr. H. Rübsamen-Waigmann; W. B. Becker; E. B. Helm; R. Brodt; H. Fischer; K. Henco; H. D. Brede


Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
916 KB
Volume
19
Category
Article
ISSN
0146-6615

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✦ Synopsis


LAV/HTLV-III/AAV viruses were isolated from 20 German patients with ARC/ AIDS in order to investigate strain variation. Virus was isolated from the peripheral blood and/or cerebrospinal fluid (CSF) in umbilical cord peripheral blood lymphocyte (PBL) cultures. Isolates were identified by their cytopathic effect (CPE), by reverse transcriptase assays on cell-free infected culture supernatant fluid (SNF), and one or more of the following: immunofluorescence assays on


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