✦ LIBER ✦

Comparative study of the isolation of human immunodeficiency virus from the lung and peripheral blood of aids patients

✍ Scribed by Dr. John R. Clarke; John D. Williamson; David M. Mitchell

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 455 KB
Volume: 39
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890390304

No coin nor oath required. For personal study only.

✦ Synopsis

HIV-1 proviral DNA was detected by the polymerase chain reaction (PCR) in leucocytes from the peripheral blood (PBL) and bronchoalveolar lavage (BAL) of 100% and 72%, respectively, of HIV-1 seropositive patients. Infective virus isolated by cocultivation was recovered from the PBL and BAL of 50% and 59% of individuals, respectively. Isolation of HIV-1 was more readily made from the lung of individuals undergoing Pneumocystis carinii pneumonia (PCP) than from patients with non-PCP lung infections. The concomitant infection of lung cells with cytomegalovirus did not affect the isolation rate of HIV-1 from the lung. HIV-1 was isolated from BAL of 23 out of 36 (64%) individuals receiving no antiviral chemotherapy and from 13 out of 24 (54%) patients who were receiving AZT.

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