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Isolation of uracil auxotrophic mutants of and their transformation with heterologous vectors

✍ Scribed by MANCZINGERX, L; ANTAL, Z; FERENCZY, L

Book ID
122114924
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
339 KB
Volume
130
Category
Article
ISSN
0378-1097

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Isolation and transformation of uracil a
Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycetePhanerochaete chrysosporium
✍ Lakshmi Akileswaran; Margaret Alic; Edith K. Clark; Jason L. Hornick; Michael H. πŸ“‚ Article πŸ“… 1993 πŸ› Springer-Verlag 🌐 English βš– 817 KB

Uracil auxotrophs of Phanerochaete chrysosporium were isolated using 5-fluoroorotate resistance as a selection scheme. The ura3 auxotrophs deficient in orotidylate decarboxylase and ura5 auxotrophs deficient in orotate phosphoribosyl transferase were characterized by enzyme assays and complementatio