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Isolation and transformation of uracil auxotrophs of the edible basidiomycete Pleurotus ostreatus

โœ Scribed by Beom-Gi Kim; Yumi Magae; Young-Bok Yoo; Suk-Tae Kwon


Book ID
109322782
Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
154 KB
Volume
181
Category
Article
ISSN
0378-1097

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Isolation and transformation of uracil a
โœ Lakshmi Akileswaran; Margaret Alic; Edith K. Clark; Jason L. Hornick; Michael H. ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 817 KB

Uracil auxotrophs of Phanerochaete chrysosporium were isolated using 5-fluoroorotate resistance as a selection scheme. The ura3 auxotrophs deficient in orotidylate decarboxylase and ura5 auxotrophs deficient in orotate phosphoribosyl transferase were characterized by enzyme assays and complementatio