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Isolation of two DNA polymerases from a mitochondrial membrane-DNA complex of mouse cell cultures

✍ Scribed by Klaus Radsak; Gerhard Seidel


Book ID
118310881
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
593 KB
Volume
71
Category
Article
ISSN
0006-291X

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Rapid isolation of mouse DNA from cells
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A rapid method for the isolation of DNA from mouse cells grown in tissue culture is described. Cells are lysed in 0.24 M sodium phosphate, pH 6.8 buffer, containing 1% sodium dodecyl sulfate, 8 M urea, and 10m3 M ethylenediaminetetraacetic acid, and the crude lysate applied to a column of hydroxyapa